研究目的
To facilitate the transition of MALDI-MS Imaging (MALDI-MSI) from basic science to clinical application, it is necessary to analyze formalin-fixed paraffin-embedded (FFPE) tissues. Our aim was to improve in-situ tryptic digestion for MALDI-MSI of FFPE samples and determine if similar results would be reproducible if obtained from different sites.
研究成果
Spatial resolution and site-to-site reproducibility can be maintained by adhering to a standardized MALDI-MSI workflow.
研究不足
The preparation of FFPE samples for MALDI-MSI is perceived as being long and complex, requiring up to two days' preparation. The spatial resolution is inferior and the time required for MALDI-MSI analysis is greater when compared to traditional microscopy.
1：Experimental Design and Method Selection:
FFPE tissues were prepared for MALDI-MSI. Samples were coated with trypsin using an automated sprayer then incubated using deliquescence to maintain a stable humid environment. After digestion, samples were sprayed with CHCA using the same spraying device and analyzed with a rapifleX MALDI Tissuetyper at 50μm spatial resolution. Data were analyzed using flexImaging, SCiLS and R.
2：Sample Selection and Data Sources:
FFPE tissues (mouse intestine, human ovarian teratoma, tissue microarray of tumor entities sampled from three different sites).
3：List of Experimental Equipment and Materials:
rapifleX MALDI Tissuetyper, TM sprayer, trypsin, CHCA matrix.
4：Experimental Procedures and Operational Workflow:
Samples were coated with trypsin using an automated sprayer, incubated using deliquescence, sprayed with CHCA, and analyzed with a rapifleX MALDI Tissuetyper.
5：Data Analysis Methods:
Data were analyzed using flexImaging, SCiLS and R.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
