研究目的
To develop a conformation-switchable smart nanoprobe for monitoring telomerase activity and enabling activity-triggered drug release in cancerous cells.
研究成果
The study successfully developed a smart nanoprobe for telomerase activity monitoring and activity-triggered drug release in cancerous cells, showing potential for cancer diagnosis and therapy. The nanoprobe's design avoids interference from cellular polymerases and reduces false positives, offering a straightforward and effective strategy for cancerous cell recognition and targeted therapy.
研究不足
The study primarily focuses on in vitro and cellular models, and the applicability in vivo or in clinical settings is not explored. The specificity and efficiency of the nanoprobe in complex biological environments need further validation.
1：Experimental Design and Method Selection:
The study designed a nanoprobe with a gold nanoparticle (AuNP) core and a dense layer of FAM-labeled hairpin DNA shell to monitor telomerase activity and trigger drug release.
2：Sample Selection and Data Sources:
HeLa, MCF-7, A549, MDA-MB-231 (cancerous cells), and L-02 (normal cells) were used. Telomerase extracts were prepared from these cells.
3：List of Experimental Equipment and Materials:
AuNPs, FAM-labeled hairpin DNA, Dox, PEG-SH, and various chemicals for cell culture and analysis.
4：Experimental Procedures and Operational Workflow:
Synthesis of AuNP-MPs, characterization, telomerase activity detection, intracellular imaging, and drug release studies.
5：Data Analysis Methods:
Fluorescence spectroscopy, confocal microscopy, flow cytometry, and ICP-OES for quantitative analysis.
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