研究目的
Developing a near-infrared ICT-based fluorescent probe for the detection of diazane with high sensitivity and selectivity, applicable in biological systems and water samples.
研究成果
The ICT-based NIR ratio probe LX was successfully developed for selective detection of diazane with high sensitivity and a wide pH range. It has practical applications in biological systems and water samples, demonstrating potential for various biological and chemical applications.
研究不足
The probe LX exhibits some cytotoxicity, and its application in living cells is limited to concentrations where cell viability is maintained.
1：Experimental Design and Method Selection:
The study involved the synthesis of a NIR ratio fluorescent sensor LX for diazane detection, utilizing intramolecular charge transfer (ICT) mechanism.
2：Sample Selection and Data Sources:
The probe's sensitivity and selectivity were tested against various biologically relevant analytes in DMSO/PBS solution.
3：List of Experimental Equipment and Materials:
Instruments included a Hitachi F-4600 fluorescence spectrophotometer, Agilent 8453 spectrophotometer, Bruker-DTX-400 spectrometer, and HPLC Q-Tof HR-MS spectrometer.
4：Experimental Procedures and Operational Workflow:
The synthesis of LX, followed by testing its fluorescence and absorption spectra changes upon addition of diazane and other analytes.
5：Data Analysis Methods:
The detection limit was determined by fluorescence titration, and the sensing mechanism was explored through ESI-MS and DFT calculations.
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