研究目的
To develop a near-infrared fluorescent probe for the simultaneous detection of H2S and GSH in living cells and zebrafish, addressing the challenge of sensing these molecules simultaneously in biological processes.
研究成果
The probe HZ-NBD demonstrated high selectivity and sensitivity for H2S and GSH detection, with applications in living cells and zebrafish. Its design offers advantages such as NIR emission, large Stokes shift, and low cytotoxicity, making it a promising tool for biological and clinical investigations.
研究不足
The probe's performance is limited to the detection of H2S and GSH, and its application in more complex biological systems or in vivo models beyond zebrafish was not explored.
1：Experimental Design and Method Selection:
The probe HZ-NBD was designed using dicyanoisophorone as the fluorophore and NBD as the response unit and quencher. The detection mechanism is based on the sulfhydryl-promoted SNAr reaction.
2：Sample Selection and Data Sources:
HepG2 cells and zebrafish were used for bioimaging.
3：List of Experimental Equipment and Materials:
UV-visible absorption spectra were determined on a Shimadzu UV-3600 spectrophotometer. Fluorescence spectra were measured on a HORIBA FL-4 Max spectrometer.
4：Experimental Procedures and Operational Workflow:
The probe's response to H2S and GSH was tested in THF/PBS mixture solution. Cell imaging was performed using confocal microscopy.
5：Data Analysis Methods:
The fluorescence intensity changes were analyzed to determine the detection limits and selectivity of the probe.
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