研究目的
To develop a nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) for SERS-based sensing that reduces blinking effects caused by thermal radiation, and to apply this system for the detection of kanamycin in various samples.
研究成果
The developed nanocomposite effectively reduces blinking effects in SERS spectra by minimizing thermal radiation. The microfluidic SERS detection system demonstrated high sensitivity and specificity for kanamycin, with a low limit of detection and potential for reuse. This system holds promise for the detection of small molecules in various samples.
研究不足
The study focuses on kanamycin detection and may not be directly applicable to other antibiotics without modification of the aptamer. The nanocomposite's stability and reproducibility under various conditions were tested, but long-term stability in real-world applications may require further investigation.
1：Experimental Design and Method Selection
The study involved the synthesis of nanocomposite structures of GO and AuNPs to reduce blinking effects in SERS spectra. The nanocomposite was characterized and applied in a microfluidic system for kanamycin detection using a specific aptamer.
2：Sample Selection and Data Sources
Kanamycin was detected in drinking water, orange juice, and milk. The aptamer was specific to kanamycin and switchable in its recognition.
3：List of Experimental Equipment and Materials
Includes graphene oxide, gold nanoparticles, Texas Red-dye conjugated Beacon aptamer, and a microfluidic detection system.
4：Experimental Procedures and Operational Workflow
The nanocomposite was synthesized and characterized. The microfluidic system was used to detect kanamycin with the aptamer, and SERS spectra were recorded.
5：Data Analysis Methods
SERS spectra were analyzed for intensity and stability. The limit of detection and linear range were determined for kanamycin.
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