研究目的
Investigating the feasibility of studying microcirculation in a lipopolysaccharide (LPS)-induced inflammation mouse model using photoacoustic microscopy (PAM).
研究成果
PAM is capable of noninvasively and continuously monitoring microcirculation changes in LPS-injected mice, showing potential for early intervention and treatment plan optimization of sepsis by investigating microcirculatory dysfunction.
研究不足
The study is limited by the single-wavelength laser used, which hinders the measurement of oxygen saturation (sO2). The measured lateral resolution is worse than the theoretical one, possibly due to optical aberration. Large sample-to-sample differences and variations in microvasculature among different regions of the mouse ear may affect the results.
1：Experimental Design and Method Selection:
The study employs PAM to noninvasively evaluate the LPS-induced inflammation model in mice, focusing on microvascular structural changes over 8 hours post-LPS application.
2：Sample Selection and Data Sources:
16 BALB/c male mice aged 10 to 12 weeks were divided into 4 groups, each receiving different LPS concentrations.
3：List of Experimental Equipment and Materials:
PAM system with a 532 nm pulsed laser, custom-made needle hydrophone, and a two-dimensional linear motorized stage for scanning.
4：Experimental Procedures and Operational Workflow:
Mice were imaged right after LPS injection and every hour for 8 hours. Microvascular quantification algorithm was used for quantitative analysis.
5：Data Analysis Methods:
Quantitative analysis of five vessel parameters (vessel density, vessel diameter, vessel ratio, vessel intensity, and vessel tortuosity) was conducted to assess changes in microvasculature.
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