研究目的
To develop sulfur and nitrogen codoped near-infrared-emitting carbon dots (NIR CDs) for efficient photothermal therapy (PTT) in mouse models, capable of being visualized by photoluminescence and photoacoustic imaging, with unique biodistribution and targeting properties for clinical biomedical applications.
研究成果
The developed sulfur and nitrogen codoped NIR CDs exhibit high photothermal conversion efficiency, excellent optical and photoacoustic imaging performance, and unique biodistribution and targeting properties, making them promising agents for clinical biomedical applications in cancer diagnosis and treatment.
研究不足
The study primarily focuses on the synthesis and preliminary in vivo testing of NIR CDs, with further clinical trials and long-term toxicity studies needed to fully assess their applicability in human treatments.
1：Experimental Design and Method Selection:
The study utilized solvothermal synthesis with dimethylsulfoxide (DMSO) as both the solvent and sulfur-doping source to prepare NIR CDs from citric acid and urea.
2：Sample Selection and Data Sources:
The CDs were characterized using TEM, AFM, EDS, XPS, FT-IR, UV–visible absorption spectra, and emission spectra. In vivo studies were conducted on mice with H22 and 4T1 tumors.
3：List of Experimental Equipment and Materials:
Equipment included an FEI Tecnai-G2-F20 transmission electron microscope, Multimode 8 instrument for AFM imaging, Inca X-Max for EDS and elemental mapping, ESCALAB MK II X-ray photoelectron spectrometer, Perkin–Elmer spectrometer for FT-IR, Shimadzu UV-3101PC spectrophotometer, Horiba Jobin Yvon Fluorolog-3 spectrometer, Olympus FV1000 confocal laser scanning microscope, cnilaser MD-655NM-HS-2W-16060512 laser, FLIR E50 thermal imaging camera, and UNT-T323 digital thermometers. Materials included citric acid, urea, DMSO, and commercially available graphene oxide.
4：Experimental Procedures and Operational Workflow:
The CDs were synthesized, characterized, and tested for photothermal performance, cytotoxicity, biodistribution, and in vivo PTT efficacy.
5：Data Analysis Methods:
Data were analyzed using ImageJ image analysis software, and statistical techniques were applied to evaluate cell viability and tumor growth rates.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
Olympus FV1000 confocal laser scanning microscope
FV1000
Olympus
Used for fluorescence imaging of cells to evaluate the cytotoxicity and photothermal ablation efficacy of the CDs.
-
cnilaser MD-655NM-HS-2W-16060512
MD-655NM-HS-2W-16060512
cnilaser
Used as a laser source for photothermal therapy and fluorescence imaging.
-
FLIR E50 thermal imaging camera
E50
FLIR Systems, Inc.
Used for capturing photothermal images to monitor temperature changes during PTT.
-
FEI Tecnai-G2-F20 transmission electron microscope
Tecnai-G2-F20
FEI
Used for TEM imaging to characterize the morphology and structure of the CDs.
-
Multimode 8 instrument
Multimode 8
Bruker Co.
Used for AFM imaging to analyze the height of the CDs.
-
Shimadzu UV-3101PC spectrophotometer
UV-3101PC
Shimadzu
Used for UV–visible absorption spectra to analyze the absorption properties of the CDs.
-
Inca X-Max instrument
Inca X-Max
Used for EDS and elemental mapping to investigate the chemical compositions of the CDs.
-
ESCALAB MK II X-ray photoelectron spectrometer
ESCALAB MK II
Used for XPS analyses to study the surface chemistry of the CDs.
-
Perkin–Elmer spectrometer
Perkin–Elmer
Used for FT-IR spectra to detect surface functional groups of the CDs.
-
Horiba Jobin Yvon Fluorolog-3 spectrometer
Fluorolog-3
Horiba Jobin Yvon
Used for emission spectra to study the fluorescence properties of the CDs.
-
UNT-T323 digital thermometers
UNT-T323
Used for measuring the photothermal effect data with a K-type thermocouple.
-
登录查看剩余9件设备及参数对照表
查看全部
