研究目的
Investigating the therapeutic effects of Ga(III) phthalocyanine as a photosensitizer in neuroblastoma cellular model for photodynamic therapy.
研究成果
Ga(III)‐Pc shows promise as a photosensitizer for PDT in neuroblastoma, with a good toxicological profile and effective intracellular accumulation. However, a fraction of cells exhibited resistance to PDT, indicating the need for further research to understand and overcome this limitation.
研究不足
The study focused on in vitro assessment, and the fraction of PDT‐resistant cells suggests potential limitations in complete tumour eradication. Further studies are needed to elucidate the mechanisms of resistance and long-term effects.
1：Experimental Design and Method Selection:
The study involved toxicological assessments, real‐time monitoring of cellular impedance, and imagistic measurements to assess the in vitro dark toxicity and PDT efficacy of Ga(III)‐Pc in SHSy5Y neuroblastoma cells.
2：Sample Selection and Data Sources:
Human neuroblastoma SH‐SY5Y adherent cell line was used, maintained in DMEM‐F12 modified medium.
3：List of Experimental Equipment and Materials:
Included Ga(III)‐Pc‐chloride, dimethylsulphoxide (DMSO), Cytotox 96 Non‐Radioactive Cytotoxicity Assay kit, CellTiter96AQueous One Solution Cell Proliferation kit, and confocal microscopy equipment.
4：Experimental Procedures and Operational Workflow:
Cells were treated with different doses of Ga(III)Pc for various time points, followed by viability, proliferation, and impedance assessments. PDT was performed using a He‐Ne laser.
5：Data Analysis Methods:
Statistical analysis was performed on triplicate experiments, with results presented as mean ± standard deviation.
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