研究目的
Investigating the therapeutic effects of a specific herbal medicine on a particular disease.
研究成果
The silicon nanowires luminescent sensor demonstrated high sensitivity and selectivity for CRP detection in saliva, with a femtomolar limit of detection and a wide operating range. This label-free sensor offers a promising tool for non-invasive cardiovascular risk assessment at home, with potential applications in preventive health care.
研究不足
The study focuses on the detection of CRP in saliva, which may not cover all biomarkers for cardiovascular diseases. The sensor's performance in real-world conditions outside controlled laboratory settings is not fully explored.
1：Experimental Design and Method Selection:
The study involves the synthesis of silicon nanowires (NWs) using a metal assisted chemical etching (MACE) approach for the development of a luminescent sensor for C-reactive protein (CRP) detection in saliva.
2：Sample Selection and Data Sources:
Commercial silicon wafers were used as the starting material for NWs synthesis. CRP and its biotinylated antibody were obtained from commercial sources.
3：List of Experimental Equipment and Materials:
Scanning electron microscopy (Zeiss, field emission Sigma microscope), HR800 Spectrometer (Horiba Jobin Yvon), Ar+ laser, UV fluorinated 60× objective, and various chemicals including hydrofluoric acid, isopropanol, gold etchant solution, and phosphate-buffered saline (PBS) buffer tablets.
4：Experimental Procedures and Operational Workflow:
The NWs were synthesized, functionalized with streptavidin and biotinylated CRP antibody, and then tested with different CRP concentrations. PL measurements were performed to assess the sensor's performance.
5：Data Analysis Methods:
The PL signals were analyzed to determine the sensor's sensitivity and selectivity towards CRP.
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scanning electron microscope
Sigma
Zeiss
Structural characterization of NWs samples
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silicon wafers
Siegert Wafer
Starting material for NWs synthesis
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Spectrometer
HR800
Horiba Jobin Yvon
Optical characterization through photoluminescence measurements
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Ar+ laser
Excitation source for PL measurements
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UV fluorinated objective
60×
Focusing the laser beam on the sample
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