研究目的
To develop a biomimetic nanoreactor (bio-NR) for synergistic chemiexcited photodynamic-starvation therapy against tumor metastasis, addressing the limitation of photodynamic therapy (PDT) in treating deeply seated metastatic tumors due to poor light penetration.
研究成果
The designed biomimetic nanoreactor (bio-NR) effectively combines chemiexcited photodynamic therapy and starvation therapy, showing excellent therapeutic effects against tumor metastasis in vitro and in vivo. This approach provides a new perspective for clinical cancer metastasis therapy.
研究不足
The study acknowledges the challenge of low intracellular H2O2 concentration limiting chemiluminescence resonance energy transfer (CRET)-based PDT. The hypoxic tumor microenvironment also poses a limitation, which was addressed by co-loading PFC for O2 supply.
1：Experimental Design and Method Selection:
The study designed a biomimetic nanoreactor (bio-NR) combining photodynamic therapy (PDT) and starvation therapy. The nanoreactor was constructed using hollow mesoporous silica nanoparticles (HMSNs) modified with photosensitizers and glucose oxidase (GOx), and encapsulated with CPPO and PFC in the cavity.
2：Sample Selection and Data Sources:
Mouse skin melanoma (B16-F10) cells were used for in vitro and in vivo experiments.
3：List of Experimental Equipment and Materials:
Materials included 1-Hexanol, cyclohexane, Triton X-100, tetraethyl orthosilicate (TEOS), HF (40%), tetrahydrofuran (THF), EDC, NHS, APTES, CPPO, Ce6, TEMPO, protease inhibitors, PFCs, and GOx. Equipment included transmission electron microscope (TEM), scanning electron microscope, confocal laser scanning microscopy (CLSM), and microplate reader.
4：Experimental Procedures and Operational Workflow:
The bio-NR was synthesized, characterized, and its therapeutic effects were evaluated in vitro and in vivo under hypoxic conditions.
5：Data Analysis Methods:
Data were analyzed using fluorescence analysis, electron spin resonance (ESR) spectroscopy, UV–vis spectra, and immunofluorescent staining.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
