研究目的
To summarize the main genetically encoded biosensors built for plant hormones, constructed using diverse components and steps of their signalling pathways.
研究成果
The review concludes that genetically encoded biosensors have significantly contributed to the understanding of plant hormone signalling. It emphasizes the potential for further development of biosensors for hormones like cytokinins, ethylene, and brassinosteroids, and the importance of reducing interference with endogenous pathways in future biosensor designs.
研究不足
The review highlights the challenges in developing biosensors that do not interfere with endogenous signalling pathways and the need for sensors that are selective, sensitive, non-invasive, fast, and quantitative. It also notes the limitations of current biosensors in terms of their influence on plant signalling networks and the complexity of constructing unimolecular FRET sensors.