研究目的
To develop and utilize a new plastic orthotopic mammary window chamber compatible with magnetic resonance imaging, nuclear imaging, and optical imaging for studying the complex microenvironment in which cancers develop, proliferate, and metastasize in small animals.
研究成果
The developed orthotopic breast cancer mammary window chamber model is compatible with multi-modality imaging, providing a powerful platform technology for therapeutic development and studies of basic cancer biology.
研究不足
The study is limited by the technical constraints of the imaging modalities used, such as the depth imaging capability of optical techniques and the spatial resolution of nuclear imaging. Additionally, the model's application is constrained to small animal studies.
1：Experimental Design and Method Selection:
Utilized rapid prototyping printer technology to construct a new plastic orthotopic mammary window chamber.
2：Sample Selection and Data Sources:
Post-breeder female SCID mice were utilized due to their immune status compatibility with human cell lines and well-developed mammary glands.
3：List of Experimental Equipment and Materials:
Objet Connex 350 rapid prototyping printer, Nikon E600 microscope with a C1 confocal attachment, Bruker Biospec 7 Tesla small-animal MRI system, Beta Imager.
4：Experimental Procedures and Operational Workflow:
MWC structures were surgically implanted over human breast cancer xenograft tumors grown in the mammary fat pads of mice.
5：Data Analysis Methods:
Optical, MR, and nuclear imaging techniques were used to analyze the tumor and surrounding microenvironment.
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