研究目的
To develop an optical label-free biosensor based on PSi Bragg reflector for studying heterogeneity in single cells through micropatterning and cell lysis assays.
研究成果
The study demonstrates the potential of PSi optical biosensors for label-free detection of biological events at single-cell resolution, highlighting sensitivity in monitoring cellular activities and the possibility to explore cell heterogeneity.
研究不足
The heterogeneity in response from single cells could be due to variability in cell contents or the method of cell rupture, not solely from cellular heterogeneity.
1：Experimental Design and Method Selection:
Photolithographic patterning of PEG hydrogel on RGD peptide-modified PSi to create micropatterns for cell adhesion and repellence.
2：Sample Selection and Data Sources:
J774 macrophage cells were used to form cell microarrays and single cell arrays on the micropatterned PSi.
3：List of Experimental Equipment and Materials:
PSi Bragg reflectors, PEGDA, photoinitiator Irgacure 2959, Milli-Q? water, lysis buffer Triton X-
4：Experimental Procedures and Operational Workflow:
1 Cells were incubated on micropatterned PSi, lysed osmotically, and the infiltration of cell lysate was monitored by reflectivity shift.
5：Data Analysis Methods:
Reflectivity spectra were collected to monitor the red shift indicating cell lysate infiltration.
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