研究目的
To develop a hypoxia-responsive mesoporous silica nanocarrier (CAGE) for enhanced cancer immunotherapy (CIT) assisted by photodynamic therapy (PDT), focusing on improving the intracellular uptake of nanocarriers and the delivery of adjuvants to dendritic cells (DCs), and exploiting PDT for the generation of immunogenic debris and recruitment of DCs in a tumor site.
研究成果
The developed hypoxia-responsive nanocarrier CAGE, combined with photodynamic therapy, significantly enhanced the recruitment and activation of dendritic cells, leading to improved antigen presentation and antitumor effects in vivo. This approach presents a promising strategy for cancer immunotherapy.
研究不足
The study does not address the potential immune response to the nanocarrier itself or the long-term stability and toxicity of the nanocarrier in vivo. Additionally, the precise mechanism of ROS-induced influx of neutrophil and its enzymatic actions for DC enrichment was not fully elucidated.
1：Experimental Design and Method Selection:
The study involved the synthesis of a hypoxia-responsive mesoporous silica nanocarrier (CAGE) doped with chlorin e6 (Ce6) and modified with azobenzene-glycol chitosan (GC)-PEG. The nanocarrier was designed to release its payload under hypoxic conditions.
2：Sample Selection and Data Sources:
B
3：F1 murine melanoma cell line and an OVA-transfected clone derived from a B16 melanoma were used. Female C57BL/6 mice were used for in vivo studies. List of Experimental Equipment and Materials:
Materials included Ce6, azobenzene-4,4’-dicarboxylic acid, CpG oligonucleotides, PEG–NH2, and Flamma-
4：Instruments included TEM, UV-vis spectrometer, spectrofluorophotometer, Zetasizer, confocal laser scanning microscopy, and flow cytometry. Experimental Procedures and Operational Workflow:
7 The synthesis of CAGE involved doping MSNs with Ce6 and modifying their surface with Azo linker, GC, and PEG. In vitro and in vivo experiments were conducted to evaluate the photodynamic effect, hypoxia-responsive behavior, and antitumor efficacy.
5：Data Analysis Methods:
Data were analyzed using flow cytometry, fluorescence microscopy, and UV/Vis spectroscopy.
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