研究目的
To develop a rapid and reliable method for the determination of mercury speciation (inorganic mercury, methylmercury, ethylmercury, and phenylmercury) in marine products using molecular spectrometry with microliquid extraction.
研究成果
The proposed method based on molecular spectroscopy offers high sensitivity, selectivity, and low cost for the speciation analysis of mercury in marine products. It provides reliable technical support for environmental monitoring and food safety standards, with satisfactory recovery rates and minimal interference.
研究不足
The method's dependence on chemical reagents and the requirement for high purity of reducing reagents may limit analysis efficiency. The stability of commonly used potassium borohydride solution necessitates same-day preparation.
1：Experimental Design and Method Selection:
The study utilized molecular spectroscopy with microliquid extraction for mercury speciation analysis. Ultrasonic extraction pretreatment was performed, followed by concentration using a C18 column and rotary evaporator. Liquid chromatography–UV digestion molecular spectrometry was used for analysis.
2：Sample Selection and Data Sources:
Marine product samples were purchased from farmers' markets. Standard solutions for mercury speciation were purchased from the National Standard Substance Center.
3：List of Experimental Equipment and Materials:
Instruments included AFS-3100 atomic fluorescence spectrometer, mercury hollow cathode lamp, Shanghai Yi Yao WX-4000 microwave digestion instrument, and Milli-Q purification system. Reagents included nitric acid, hydrochloric acid, potassium borohydride, and standard mercury solutions.
4：Experimental Procedures and Operational Workflow:
Samples were digested using a microwave digestion instrument, followed by adjustment of pH and passage through a modified C18 column for mercury concentration. The eluent was evaporated and concentrated before analysis.
5：Data Analysis Methods:
The signal values of each component were recorded and averaged over seven measurements to investigate the influence of UV digestion and potassium borohydride concentration.
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