研究目的
To develop a novel bone-implant material with antibacterial property and osteogenic promotion utilizing red phosphorus and near-infrared light.
研究成果
The Ti-RP-IR780-RGDC bone implant shows good biostability and biocompatibility with excellent antibacterial property under NIR irradiation and promotes osteogenesis, making it a promising material for clinical orthopedics.
研究不足
The study focuses on the synergistic effect of photothermal therapy and photodynamic therapy for antibacterial performance and osteogenic promotion, but the long-term stability and biocompatibility of the coating in vivo require further investigation.
1：Experimental Design and Method Selection:
The study involved the preparation of a red phosphorus/IR780/arginine-glycine-asparticacid-cysteine (RGDC) coating on titanium bone-implant. The methodology included photothermal therapy (PTT) and photodynamic therapy (PDT) for antibacterial performance and osteogenic promotion.
2：Sample Selection and Data Sources:
Medical-grade pure Ti plates and rods were used as substrates. Red phosphorus (RP), IR780, and RGDC peptide were purchased from commercial sources.
3：List of Experimental Equipment and Materials:
Equipment included a tube furnace, ultrasonic cleaner, scanning electronic microscopy (SEM), transmission electron microscopy (TEM), thermal imager, microplate reader, and fluorescence microscope.
4：Experimental Procedures and Operational Workflow:
The process involved hydrothermal treatment of RP, coating preparation on Ti plates, characterization of the coating, evaluation of photothermal performance, in vivo antibacterial ability tests, and histological and Micro-CT evaluation.
5：Data Analysis Methods:
Statistical significance was evaluated using a one-way analysis of variance (ANOVA) program and a student t-test.
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SEM
JSM-6510LV
JEOL
Examination of surface morphologies
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Fluorescence microscope
IX73
Olympus
Capturing images of stained tissues
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Ti plates
6 mm diameter, 2 mm thick
Fu-Tai Metal Materials Co.
Substrates for the bone-implant material
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Red phosphorus
Sinopharm Chemical Reagent Co.
Photothermal agent
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IR780
Sigma-Aldrich
Photosensitizer to generate ROS
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RGDC peptide
GL Biochem (Shanghai) Ltd.
Promoting osteogenesis
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TEM
Tecnai G20
Obtaining transmission electron microscopy images
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Thermal imager
MSX
FLIR Systems Inc
Recording temperature
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Microplate reader
SpectraMax i3
Molecular Devices
Evaluation of ROS generation
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CT
SkyScan 1176
2D scanning of tibias
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