研究目的
Developing highly selective and sensitive methods for hydrazine detection, especially in living organisms.
研究成果
A new high selective fluorescent probe for hydrazine was developed with a large fluorescence change ratio, enabling powerful molecular tool for hydrazine detection in living cells with negligible cytotoxicity.
研究不足
The study focuses on in vitro detection and imaging in HeLa cells; further in vivo applications and broader biological samples were not explored.
1：Experimental Design and Method Selection:
The probe was designed based on benzothiazole substituted naphthalene derivative with acetyl as the recognizing site.
2：Sample Selection and Data Sources:
HeLa cells were used for cytotoxicity and imaging experiments.
3：List of Experimental Equipment and Materials:
Spectrophotometers, NMR spectrometer, mass spectrometer, confocal laser scanning microscope.
4：Experimental Procedures and Operational Workflow:
Synthesis of the probe, spectral measurements, cell viability assay, and cell imaging.
5：Data Analysis Methods:
Fluorescence intensity changes were analyzed to determine hydrazine detection limits and selectivity.
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