研究目的
Developing a fast response two-photon fluorogenic probe based on Schiff base derivatives for monitoring nitric oxide levels in living cells and zebrafish.
研究成果
The developed two-photon small molecular NO fluorogenic probe based on Schiff base derivatives responds to NO very quickly (o50 s) with high specificity. It can visualize images of endogenous and exogenous NO in SH-SY5Y cells as well as in zebrafish under two-photon excitation. This new detecting mechanism with a fast response towards NO may provide a new strategy for the design of TP fluorogenic probes and further application in the early diagnosis of disease.
研究不足
The study does not mention any specific limitations.
1：Experimental Design and Method Selection:
The study designed a novel two-photon fluorogenic probe (XNO1) based on Schiff base derivatives for NO detection. The probe's response to NO was evaluated through photophysical properties, kinetics experiments, and specificity tests.
2：Sample Selection and Data Sources:
SH-SY5Y cells and zebrafish were used as biological samples to assess the probe's ability to monitor NO levels.
3：List of Experimental Equipment and Materials:
The probe XNO1 was synthesized and characterized using 1H NMR, 13C NMR, and HRMS spectra. Fluorescence microscopy was used for imaging.
4：Experimental Procedures and Operational Workflow:
The probe's photophysical properties towards NO were investigated. The ability to detect exogenous and endogenous NO in living cells and zebrafish was assessed using one- and two-photon fluorescence microscopy.
5：Data Analysis Methods:
Fluorescence intensity changes were recorded and analyzed to determine the probe's response to NO.
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