研究目的
Developing a highly selective "turn-on" fluorescent probe for detecting Cu2+ in two different sensing mechanisms.
研究成果
The developed fluorescent probe (1) is highly selective for Cu2+ detection in two different mechanisms: chelation-controlled C=N isomerisation in anhydrous acetonitrile and Cu2+-promoted cyclization reaction in aqueous acetonitrile. The probe was successfully applied for intracellular Cu2+ imaging, demonstrating its potential for biological and environmental monitoring applications.
研究不足
The study's limitations include the necessity of specific conditions (anhydrous acetonitrile or aqueous acetonitrile) for the probe's operation and the potential for interference from other metal ions in complex samples.
1：Experimental Design and Method Selection:
The study involved the synthesis of a coumarin-derived hydrazone (1) and its application as a fluorescent probe for Cu2+ detection in anhydrous acetonitrile and aqueous acetonitrile. The fluorescence response was attributed to chelation-controlled C=N isomerisation and Cu2+-promoted cyclization reaction, respectively.
2：Sample Selection and Data Sources:
The probe's performance was tested with various metal ions to assess selectivity and sensitivity.
3：List of Experimental Equipment and Materials:
Instruments included a Bruker Av400 NMR spectrometer, Bruker Esquire HCT mass spectrometer, Hitachi F-7000 fluorescence spectrometer, and Olympus FV1000 confocal laser scanning microscope.
4：Experimental Procedures and Operational Workflow:
The synthesis of 1 and 2, fluorescence spectra measurement, and cell imaging were detailed.
5：Data Analysis Methods:
Fluorescence titration, time-dependent fluorescence responses, and pH-dependent fluorescence responses were analyzed.
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