研究目的
To introduce and demonstrate the use of visible-near infrared spectral imaging for non-destructive monitoring of biological materials, specifically wood moisture content during drying and leaf water stress using time-resolved fluorescence images.
研究成果
Vis-NIR spectral imaging is effective for non-destructive monitoring of biological materials, as demonstrated in wood moisture content and leaf water stress applications. However, it primarily captures surface information, and internal details require further investigation, especially with transmittance methods hindered by scattering. Future work should focus on clarifying light pathways and improving techniques for internal monitoring.
研究不足
Measurements are based on reflectance and emission from sample surfaces and may not reflect internal information; scattering in biological materials complicates transmittance measurements; chlorophyll fluorescence induction is affected by various factors, making it difficult to isolate specific stresses without considering multiple parameters.
1:Experimental Design and Method Selection:
The study employs hyperspectral imaging and time-resolved fluorescence imaging combined with multivariate analysis techniques such as partial least squares regression (PLSR) and principal component analysis (PCA) for non-destructive monitoring.
2:Sample Selection and Data Sources:
For wood monitoring, European beech and Scots pine samples (10 total) with specific dimensions were used, immersed in water and air-dried. For leaf stress monitoring, potted Epipremnum aureum plants were used under controlled conditions with water stress applied.
3:List of Experimental Equipment and Materials:
Hyperspectral camera (Hyspex VNIR-1600, Norsk Elektro Optikk), CCD video camera module (XC-ST50, Sony), band-pass filter, excitation lights at 470 nm and 660 nm, thermostatic chamber.
4:Experimental Procedures and Operational Workflow:
For wood, hyperspectral images were acquired seven times during drying, with weight measurements; PLSR models were built. For leaves, time-resolved CFI images were captured at 3 frames per second for 20s after light irradiation, and PCA was applied.
5:Data Analysis Methods:
PLSR for moisture content prediction, PCA for analyzing time dependency of fluorescence intensity, with metrics like RPD for accuracy.
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