研究目的
To develop and clinically test a navigation-guided fiberoptic Raman probe integrated with a brain biopsy needle for in situ interrogation of brain tissue to improve diagnostic yield in brain biopsies.
研究成果
The novel Raman spectroscopy probe was successfully integrated into the surgical workflow, allowing seamless in situ tissue interrogation with high spectral quality in both fingerprint and high-wavenumber regions. The spectra exhibited expected molecular features and were consistent with other in vivo and ex vivo measurements, demonstrating the technology's robustness. Further clinical development is planned to gather more data for real-time tissue classification models.
研究不足
The probe design introduced artifacts from MgF? prism at 1300 and 1615 cm?1, which could interfere with important Raman features. The study had a small sample size (3 patients), limiting statistical power. Differences in acquisition parameters between systems (e.g., spot diameter, integration time) may affect signal comparability. Future developments need to address sterilization and artifact issues for broader clinical utility.
1：Experimental Design and Method Selection:
The study involved designing a Raman spectroscopy probe integrated into a commercial brain biopsy needle to operate in both fingerprint (800-1600 cm?1) and high-wavenumber (2800-3600 cm?1) spectral regions. The probe was validated in vivo during human brain biopsy procedures to assess its integration into surgical workflow and signal quality.
2：Sample Selection and Data Sources:
Three brain tumor patients eligible for stereotactic brain biopsy were selected with institutional ethics approval. Raman spectra were acquired from normal brain and tumor tissues based on pre-operative MRI and neuronavigation guidance.
3：List of Experimental Equipment and Materials:
The probe consists of a central illumination fiber (100 μm core diameter) surrounded by twelve collection fibers made of low-OH step-index silica fibers (numerical aperture
4：22), with short-pass and high-pass filters (EMVision LLC), a MgF? prism, and a heat-shrink sleeve for sterilization. Lasers at 671 nm (Laser Quantum) and 785 nm (Innovative Photonics Solutions) were used for excitation, and a CCD detector (ANDOR Technology) with a holographic diffraction grating for detection. A neuronavigation system (Stealth Station:
Medtronic) was used for needle positioning.
5：Experimental Procedures and Operational Workflow:
The probe was sterilized using STERRAD gas sterilization. During surgery, the probe was inserted into the biopsy needle, aligned with the distal opening, and guided by neuronavigation to target positions. Raman measurements were taken at various points along the needle trajectory, followed by tissue extraction via negative pressure and rotation of the inner cannula. Spectra were acquired with automatic exposure control (0.005-0.3 s integration time, 20 mW laser power).
6：005-3 s integration time, 20 mW laser power).
Data Analysis Methods:
5. Data Analysis Methods: Raw spectra were processed using custom Python scripts: background subtraction, system response correction using a NIST standard (SRM2241), polynomial fitting for autofluorescence removal, Savitsky-Golay smoothing, and standard variate normalization (SNV).
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