研究目的
To quantify OCTA signal changes at the level of the choriocapillaris in patients with different stages of central serous chorioretinopathy and to explore any correlation between subretinal fluid and retinal pigment epithelium alterations and the OCTA signal.
研究成果
Patients with active CSC show significantly increased and decreased OCTA signals in the choriocapillaris compared to inactive CSC and controls. The decreased signal in subretinal fluid areas may indicate hypoperfusion or artifacts, while increased signals in RPE atrophy areas suggest possible displacement of deeper choroid layers. The lack of changes in altered RPE areas argues against primary choriocapillaris injury. Further studies with improved imaging are necessary to confirm these findings.
研究不足
The study has a retrospective design, a relatively small number of patients in some subgroups, and potential artifacts from subretinal fluid, pigment epithelial detachments, or photoreceptor irregularities that could affect OCTA signals. The use of averaged pixel intensity for quantification may lead to over- or underestimation of flow changes. Higher resolution images and optimized acquisition techniques are needed for future validation.
1：Experimental Design and Method Selection:
A retrospective study design was used. OCTA images were obtained using the Zeiss AngioPlex system, and an image-processing algorithm programmed in R language was employed for quantification. Spatial correlation analysis involved overlapping macular edema heatmaps and fundus autofluorescence images with OCTA images.
2：Sample Selection and Data Sources:
101 eyes from 78 CSC patients and 42 eyes from 34 healthy controls were included. Patients were classified into four CSC subgroups based on Daruich et al.'s classification. Data were collected from patient charts at the Eye Center of the University of Freiburg between December 2015 and April
3：List of Experimental Equipment and Materials:
20 Zeiss AngioPlex OCTA system, fundus autofluorescence imaging equipment, software for image analysis (R language, GraphPad Prism 6).
4：6). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: OCTA images were acquired with a 6x6 mm volume scan. The choriocapillaris layer was visualized using a 30-μm slab below the inner RPE. Images were processed with Gaussian blur and morphologic hdome operator, and pixels with increased or decreased gray-scale values were color-coded. Shadowing artifacts were manually removed. Spatial correlation was analyzed by aligning images and outlining regions of interest.
5：Data Analysis Methods:
Statistical analysis was performed using GraphPad Prism 6. Non-parametric tests (Mann-Whitney U test for two groups, ANOVA/Bonferroni for multiple groups) were used due to non-normal data distribution. Significance was set at p < 0.05.
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