研究目的
To understand and quantify the effect of microwell surface coatings on signal intensity of small organic molecules of clinical, environmental and forensic interest in LDTD-QqQMS.
研究成果
Chelating agents like EDTA and NTA as microwell coatings significantly enhance signal intensity in LDTD-QqQMS by promoting the formation of uniform, thin nanocrystal layers that are easier to desorb, rather than reducing chemisorption. NTA showed better performance for some compounds. Future research should focus on morphology-ionization links and high-resolution mass spectrometry applications.
研究不足
The study did not fully explain the selectivity of coatings for certain chemical functions. The effect of coatings in real matrices was reduced due to interference from organic matter. Further studies with high-resolution mass spectrometry are needed to identify ions and understand ionization effects.
1：Experimental Design and Method Selection:
The study used LDTD-QqQMS to analyze the effect of various surface coatings (chelating agents and PTFE) on signal enhancement. XPS and SEM were employed to study surface composition and morphology.
2：Sample Selection and Data Sources:
Target analytes included pesticides, pharmaceuticals, hormones, and other small organic molecules. Real matrices (surface water, urine, plasma) were extracted and spiked.
3：List of Experimental Equipment and Materials:
LDTD source (Phytronix T-960), triple quadrupole mass spectrometer (ThermoFisher TSQ Vantage), XPS spectrometer (Kratos Ultra DLD), SEM (Hitachi FEG-SEM S-4700), chemicals from Sigma-Aldrich and Fisher Scientific.
4：Experimental Procedures and Operational Workflow:
Microwell plates were coated with chelating agent solutions, analytes deposited, dried, and analyzed by LDTD-QqQMS. XPS and SEM analyses were conducted on coated and uncoated surfaces.
5：Data Analysis Methods:
Peak areas from MRM transitions were used; ANOVA and Tukey's HSD tests were applied for statistical analysis. Data processed with XCalibur software.
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