研究目的
To compare in vivo osteogenesis on rough threaded dental implants with and without calcium phosphate (CaP) coating deposition, alone or in association with low-level laser therapy (LLLT) by gallium aluminum arsenide.
研究成果
Both calcium phosphate coating and low-level laser therapy, individually or combined, enhance early bone formation and implant fixation in short-term healing. They are effective, low-cost methods that should be considered in clinical practice to improve osseointegration, particularly in cases requiring accelerated healing.
研究不足
The study is limited to an animal model (rabbits), which may not fully translate to human clinical scenarios. The short evaluation periods (up to 6 weeks) may not capture long-term effects. The sample size (n=5 per group per time point) might be small for broader generalizations. Potential variations in individual animal responses were not accounted for.
1：Experimental Design and Method Selection:
The study used an in vivo animal model with rabbits to evaluate bone formation and implant fixation. Four groups were defined based on treatments: control implant, CaP-coated implant, implant with LLLT, and implant with both CaP coating and LLLT. Histomorphometric analysis and removal torque tests were employed to assess bone-implant contact and mechanical fixation.
2：Sample Selection and Data Sources:
Twenty male New Zealand rabbits were used, with implants placed in their tibias. Samples included 80 rough threaded dental implants, half treated with CaP coating via biomimetic method.
3：List of Experimental Equipment and Materials:
Equipment included scanning electron microscopy (SEM) (LEO 435 VPI), energy dispersion spectrophotometry (EDS) (Oxford model: 7059), low-level laser therapy device (GaAlAs: Photon Laser III; DMC), digital torque wrench (Mark-10 Co.), optical microscope (Axioplan 2, Carl Zeiss), and software (ImageJ v1.31). Materials included titanium grade 2 implants, chemicals for CaP coating, anesthetics (xylazine chloride, ketamine chloride), and surgical supplies.
4：31). Materials included titanium grade 2 implants, chemicals for CaP coating, anesthetics (xylazine chloride, ketamine chloride), and surgical supplies.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Implants were characterized using SEM and EDS before surgery. Rabbits were anesthetized, and implants were surgically placed in tibias. LLLT was applied transcutaneously at specific doses for 7 days. Euthanasia was performed at 1, 2, and 6 weeks for histological analysis and at 6 weeks for removal torque tests. Bone samples were processed, embedded, and analyzed microscopically.
5：Data Analysis Methods:
Quantitative data were analyzed using one-way ANOVA with Tukey post hoc test for multiple comparisons. Statistical significance was set at P < 0.05.
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