1：Experimental Design and Method Selection:

The study involved synthesizing star-shaped copolymers via RAFT polymerization to create thermosensitive materials with adjustable LCSTs for targeted drug delivery in photodynamic therapy. Theoretical models included polymer chemistry principles for copolymer synthesis and characterization.

2：Sample Selection and Data Sources:

Samples included synthesized copolymers with varying molecular weights, characterized using spectroscopic and chromatographic techniques. Data were obtained from laboratory experiments.

3：List of Experimental Equipment and Materials:

Equipment included NMR spectrometer, FTIR spectrometer, GPC system, UV-Vis spectrophotometer, DLS analyzer, fluorescence spectrophotometer, and MTT assay tools. Materials included NIPAM, OEGMA, DMP, AIBN, DMAP, DCC, THPP, and cell culture reagents.

4：Experimental Procedures and Operational Workflow:

Synthesis involved RAFT polymerization steps, purification by dialysis, and characterization of copolymers. Micelle formation was studied using fluorescence and DLS. Cytotoxicity and phototoxicity were assessed via MTT assays on HeLa and L929 cells.

5：Data Analysis Methods:

Data were analyzed using GPC for molecular weight, UV-Vis for LCST determination, fluorescence for CMC and singlet oxygen quantum yield, and statistical methods for cell viability assays.