研究目的
To develop a simple and sensitive derivative spectrophotometric method for the determination of Fe(II) and Ru(III) using 1,10-phenanthroline as a complexing agent.
研究成果
The developed second derivative spectrophotometric method is simple, sensitive, and reliable for determining Fe(II) in pharmaceutical and food samples and Ru(III) in synthetic mixtures. It offers good accuracy, precision, and is environmentally friendly due to the use of water as a solvent. The method is suitable for routine analysis.
研究不足
The method may be affected by interference from certain ions (e.g., F-, Cl-, and transition metals) at higher concentrations. It requires specific pH conditions and heating for Ru(III) determination, which could be time-consuming. The sensitivity range is limited to 0.1-2.0 μg mL-1 for Fe(II) and 5.0-20.0 μg mL-1 for Ru(III).
1：Experimental Design and Method Selection:
The method uses second derivative spectrophotometry with baseline-to-peak measurement techniques for determining Fe(II) and Ru(III) concentrations. Complex formation with 1,10-phenanthroline at specific pH levels is employed.
2：Sample Selection and Data Sources:
Samples include pharmaceutical formulations (e.g., Livogen-XT, Conviron-TR, Orofer XT tablets) for Fe(II) and synthetic mixtures for Ru(III). Rice samples were also analyzed for Fe(II).
3：List of Experimental Equipment and Materials:
UV-visible Spectrophotometer (Hitachi Model 1800), 10 mm matched quartz cells, ferrous ammonium sulfate, ruthenium trichloride hexahydrate (Merck India Ltd.), o-phen monohydrate (S.D. Fine Chem Mumbai, India), hydroxylamine hydrochloride, acetic acid, sodium acetate, ethanol, distilled water.
4：Experimental Procedures and Operational Workflow:
For Fe(II), transfer standard solutions to volumetric flasks, add o-phen and pH 5 buffer, dilute, and measure absorbance at 535 nm. For Ru(III), add hydroxylamine, o-phen, and pH 4 buffer, heat on water bath, cool, dilute, and measure at 466 nm. Calibration curves are constructed.
5：Data Analysis Methods:
Beer's law is applied for linear range determination. Correlation coefficients and regression equations are calculated. Precision, accuracy, recovery studies, and interference tests are conducted.
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