1：Experimental Design and Method Selection:

The study involved designing and synthesizing a CLP (Ce6-luminol-PEG) conjugate capable of bioluminescence resonance energy transfer (BRET) for imaging and therapy. Methods included chemical synthesis, spectroscopic characterization, and in vitro and in vivo testing.

2：Sample Selection and Data Sources:

Samples included the CLP conjugate, various cell lines (e.g., A549, B16F10, MCF-7, RAW264.7, MOVAS), and animal models (mice with peritonitis, acute liver injury, ulcerative colitis, and A549 xenografts). Data were acquired through laboratory experiments.

3：7, MOVAS), and animal models (mice with peritonitis, acute liver injury, ulcerative colitis, and A549 xenografts). Data were acquired through laboratory experiments. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment included transmission electron microscopy (TEM), UV-visible spectrometer, fluorescence spectrometer, IVIS Spectrum imaging system, confocal microscope, flow cytometer, HPLC system, and Western blot apparatus. Materials included Ce6, luminol, PEG, H2O2, MPO, cell culture reagents, and animal models.

4：Experimental Procedures and Operational Workflow:

Synthesis of CLP conjugate, characterization of its properties, in vitro luminescence and cytotoxicity tests, in vivo imaging in mouse models, antitumor efficacy studies, and safety assessments. Procedures involved specific protocols for each assay, such as incubation times, dosages, and imaging parameters.

5：Data Analysis Methods:

Data were analyzed using statistical methods (e.g., one-way ANOVA, Student's t-test) with software like SPSS. Quantitative analysis of images was performed with manufacturer-provided software.