研究目的
To develop a simple, low-cost, and sensitive luminescent sensor for detecting disaccharides (sucrose, maltose, lactose) in food products using a water-soluble rod-coil ionic block copolymer.
研究成果
The PF-b-PDI copolymer with HQ serves as an effective fluorescent probe for sensitive and selective detection of sucrose, maltose, and lactose in food, with low detection limits and good applicability to real milk samples, showing minimal deviation from standard methods.
研究不足
The method requires specific enzymes for each disaccharide, which may add complexity and cost. It is sensitive to interference from other components in real samples, though selectivity was demonstrated. The reaction time is 60 minutes, which might not be rapid enough for some applications.
1：Experimental Design and Method Selection:
The study utilized a fluorescence quenching mechanism based on the enzymatic generation of H2O2 from disaccharides, which quenches the fluorescence of the PF-b-PDI copolymer in the presence of hydroquinone (HQ).
2：Sample Selection and Data Sources:
Disaccharide solutions (sucrose, maltose, lactose) and milk samples were used. Chemicals were purchased from Sigma-Aldrich and Solarbio.
3：List of Experimental Equipment and Materials:
PF-b-PDI copolymer, hydroquinone (HQ), hydrogen peroxide (H2O2), glucose oxidase (GOx), sucrase, maltase, lactase, disaccharides, milk. Equipment included a Hitachi F-4600 fluorescent spectrophotometer.
4：Experimental Procedures and Operational Workflow:
For each disaccharide, specific concentrations of PF-b-PDI, HQ, enzymes (GOx and disaccharidase), and disaccharide were mixed and incubated at 37°C for 60 minutes, followed by fluorescence measurement at excitation 386 nm and emission 422 nm.
5：Data Analysis Methods:
Fluorescence intensity was measured, with F0 as intensity without disaccharide and F with disaccharide. Calibration curves were plotted, and detection limits were calculated based on signal-to-noise ratio (S/N=3).
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fluorescent spectrophotometer
F-4600
Hitachi
Used to measure fluorescence intensity and spectra of the solutions during disaccharide detection experiments.
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glucose oxidase
Sigma-Aldrich
Enzyme used to catalyze the oxidation of glucose to generate hydrogen peroxide (H2O2) in the detection process.
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sucrase
Solarbio
Enzyme used to catalyze the hydrolysis of sucrose to glucose.
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maltase
Solarbio
Enzyme used to catalyze the hydrolysis of maltose to glucose.
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lactase
Solarbio
Enzyme used to catalyze the hydrolysis of lactose to glucose.
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hydroquinone
Sigma-Aldrich
Reductant used to enable fluorescence emission of PF-b-PDI, which is oxidized by H2O2 to cause quenching.
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hydrogen peroxide
Sigma-Aldrich
Oxidant used to quench the fluorescence of PF-b-PDI by oxidizing hydroquinone.
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