研究目的
Exploring the possible mechanisms of liver injury induced by CdS nanoparticles and investigating whether nanoparticles of different sizes induce toxicity in mice.
研究成果
CdS nanoparticles induce oxidative damage and inflammatory responses in the liver, with toxicity being size-dependent; smaller nanoparticles (80-100 nm) cause more severe effects than larger ones (110-130 nm). This suggests that nanoparticle size is a critical factor in hepatotoxicity, and the findings provide insights for disease prevention and control related to nanomaterial exposure.
研究不足
The study used only male mice, limiting generalizability to females. The sample size was small (10 mice per group), and the route of exposure (intraperitoneal injection) may not fully represent environmental or occupational exposure scenarios. Further research is needed to confirm mechanisms and assess long-term effects.
1：Experimental Design and Method Selection:
The study involved treating male mice with CdS nanoparticles of two different sizes (110-130 nm and 80-100 nm) via intraperitoneal injection to assess liver toxicity. Methods included measuring oxidative stress markers (SOD, GSH, MDA), cadmium content, hematological indexes, liver function indicators, and histopathological examination.
2：Sample Selection and Data Sources:
Thirty specific-pathogen-free ICR male mice were used, randomly divided into three groups (control, 110-130 nm CdS, 80-100 nm CdS). Data were collected from blood and liver tissue samples.
3：List of Experimental Equipment and Materials:
Equipment included ICP-MS analyzer (Agilent 7500 type), homogenizer (FA25, FLUKO), transmission electron microscope (Hitachi H-7650), and assay kits for SOD, GSH, MDA. Materials included CdS nanoparticles synthesized in-house, chemicals like cadmium chloride, thiourea, ethylenediamine, cadmium acetate, L-cysteine, ethanol amine, and assay kits from Nanjing Jiancheng Bioengineering Research Institute.
4：Experimental Procedures and Operational Workflow:
Mice were injected with CdS NPs or saline every other day for 15 times. After sacrifice, blood and liver tissues were collected for analysis of cadmium content, oxidative stress markers, hematology, liver function, and histopathology. Tissues were homogenized, centrifuged, and analyzed using specified kits and instruments.
5：Data Analysis Methods:
Data were analyzed using one-way ANOVA with post-hoc tests (LSD or Dunnett's T3) in SPSS version 16.0, with significance set at p < 0.05.
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