研究目的
To develop a facile synthetic method for multi-color fluorescent carbon dots from tomato and evaluate their performance for Fe3+ ion sensing and bioimaging applications.
研究成果
The study successfully fabricated multi-color fluorescent carbon dots from tomato with high selectivity for Fe3+ ion detection and excellent biocompatibility for cell imaging. Blue-CDs showed the best sensitivity with a low LOD of 0.016 μM. The method is green, cost-effective, and applicable to real samples like biofluids and pharmaceuticals, with potential for biomedical uses.
研究不足
The quantum yields of the green and yellow CDs are lower than that of blue CDs (4.21% and 2.76% vs. 12.70%), which may affect sensitivity in some applications. The method requires specific acid treatments and dialysis, which could be optimized for scalability. Selectivity was tested against common ions and biomolecules, but not all possible interferents in complex samples.
1：Experimental Design and Method Selection:
A green synthetic approach using chemical oxidation with H2SO4 and H3PO4 to fabricate blue, green, and yellow carbon dots from tomato. The method is designed for simplicity and environmental friendliness.
2：Sample Selection and Data Sources:
Tomato (Solanum lycopersicum fruit) purchased from a local market in Seoul, Korea. Metal salts, acids, and other chemicals procured from suppliers like DaeJung Chemicals, Junsei Chemical, and Sigma-Aldrich.
3：List of Experimental Equipment and Materials:
Spectra/Por dialysis membrane (MWCO 1000 Da), deep freezer, sonicator, heating equipment, UV/vis spectrophotometer, fluorescence spectrometer, FT-IR spectrometer, zeta potential analyzer, XPS, HR-TEM, XRD, confocal laser scanning microscope, MTT assay kit.
4：Experimental Procedures and Operational Workflow:
Freeze tomato slices, treat with acids (H2SO4 for blue-CDs, H3PO4 for green and yellow-CDs) at specific temperatures and times, neutralize with NaOH, dialyze against DI water, characterize optical properties, test for Fe3+ ion sensing selectivity and sensitivity, perform cell imaging and cytotoxicity assays.
5：Data Analysis Methods:
Fluorescence intensity measurements, calibration curves for Fe3+ concentration, statistical analysis for recoveries and standard deviations, imaging analysis for cell uptake.
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