研究目的
Design and production of new types of fluorescent molecular rotors suitable for visualization in various biological systems and environments, with the aim of monitoring photodynamic processes in real time and designing more effective photosensitizing agents.
研究成果
The synthesized supramolecular triads exhibit reduced fluorescence intensity compared to the parent compound, with quenching dependent on ligand structure. The triad with tyrosine shows dual functionality as a fluorescence sensor in low glycerol concentrations and a molecular rotor in higher viscosity ranges, making it promising for environmental viscosity probing and biological applications. Future work could explore additional ligands and in vivo studies.
研究不足
The study is limited to specific axial ligands and buffer-glycerin solvent systems; it may not generalize to other ligands or environments. The quantum-chemical simulations use approximations (e.g., B3LYP/3-21G basis set), which might not capture all electronic effects. Experimental conditions (e.g., solvent purity, temperature control) could introduce variability.
1：Experimental Design and Method Selection:
The study involved synthesizing supramolecular triads of Sn(IV)-porphyrin with axial ligands, characterizing their structures using spectroscopic and quantum-chemical methods, and investigating their fluorescent properties in varying viscosity media. Theoretical models included DFT with B3LYP functional for quantum-chemical simulations.
2：Sample Selection and Data Sources:
Commercially available compounds such as 5,10,15,20-tetra(4-sulfophenyl)porphyrin, L-tyrosine, and 2-(2-hydroxyphenyl)benzoxazole from Sigma-Aldrich were used. Samples were synthesized and purified via column chromatography.
3：List of Experimental Equipment and Materials:
Equipment included a Bruker Avance III 500 NMR spectrometer for NMR studies, a Cary 300 spectrophotometer (Agilent, USA) for UV-Vis spectra, and a RF 5301PC spectrofluorimeter (Shimadzu, Japan) for fluorescence measurements. Materials included aluminum oxide for chromatography, solvents like water, ethanol, DMF, and phosphate buffer-glycerin mixtures.
4：Experimental Procedures and Operational Workflow:
Synthesis involved refluxing SnP(OH)2 with ligands in water or DMF, followed by purification. NMR experiments were conducted with specific parameters (e.g., 500.17 MHz, spectral windows 20 ppm). Fluorescence spectra were recorded at 416 nm excitation, with slit widths of 5 nm, in phosphate buffer (pH 7.4) at 1?10^-6 mol/L concentration. Quantum yields were calculated using a standard method.
5：17 MHz, spectral windows 20 ppm). Fluorescence spectra were recorded at 416 nm excitation, with slit widths of 5 nm, in phosphate buffer (pH 4) at 1?10^-6 mol/L concentration. Quantum yields were calculated using a standard method.
Data Analysis Methods:
5. Data Analysis Methods: Data analysis included interpretation of NMR spectra (1D and 2D), UV-Vis and fluorescence spectral analysis, and quantum-chemical calculations using DFT. Statistical analysis involved comparing self-diffusion coefficients from DOSY and calculating quantum yields.
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