研究目的
To develop a novel method for encapsulating zinc phthalocyanine aggregates into bovine serum albumin aggregates for potential applications in photodynamic therapy, photothermal therapy, and drug delivery systems.
研究成果
ZnPc aggregates are selectively encapsulated into dimeric BSA aggregates, not monomeric BSA, due to a larger hydrophobic cavity. This encapsulation results in H-aggregation of ZnPc, exhibiting unique fluorescence in the near-IR region. The findings suggest potential for improved drug delivery systems leveraging the EPR effect for photodynamic and photothermal therapies.
研究不足
The gel-filtration chromatography may not clearly distinguish between dimeric and larger oligomeric forms of BSA due to column limitations. The presence of Triton X-100 in the complex could affect measurements, and the method's applicability to other hydrophobic drugs needs further validation.
1：Experimental Design and Method Selection:
The study aimed to encapsulate ZnPc aggregates into BSA aggregates using gel-filtration chromatography and spectroscopic methods to analyze the hybridization.
2：Sample Selection and Data Sources:
ZnPc was synthesized as previously described, and BSA (fatty acid-depleted, purity ≥96%) was purchased from Aldrich Co.
3：List of Experimental Equipment and Materials:
Instruments included a JASCO V-570 spectrometer for electronic absorption spectra, a JASCO J-725 spectrodichrometer for circular dichroism spectra, a monochromator (JASCO CT-25CP) and photomultiplier (Hamamatsu Photonics R5509-42) for fluorescence spectra, and a Sephadex G-100 column for gel-filtration chromatography. Materials included ZnPc, BSA, Triton X-100, glass beads, triphenylphosphine (PPh3), Tris-HCl buffer, NaCl, and ultrafiltration membranes.
4：Experimental Procedures and Operational Workflow:
ZnPc was complexed with Triton X-100 in water, sonicated for 30 min, then stirred with BSA for 3 h. The product was purified by ultrafiltration and membrane filtration. Gel-filtration chromatography was performed on a Sephadex G-100 column with Tris-HCl buffer at pH 8 and 1 M NaCl, monitoring elution volumes and absorption spectra. Spectroscopic measurements were conducted to analyze electronic absorption, circular dichroism, and fluorescence.
5：Data Analysis Methods:
Data were analyzed using spectroscopic techniques to identify peaks and interactions, with exciton splitting calculated from spectral data.
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