1：Experimental Design and Method Selection:

The study used New Zealand white rabbits divided into groups with controlled light/dark cycles and yellow filter exposure to investigate the effect on intraocular pressure (IOP) and melatonin levels. IOP was measured using a TonoVet contact tonometer, and melatonin levels were analyzed via HPLC. Pharmacological antagonists were applied to block melatonin receptors and melanopsin.

2：Sample Selection and Data Sources:

Male New Zealand white rabbits weighing 2.5 ± 0.5 kg were used. They were kept in individual cages with free access to food and water, under 12h light/dark cycles or with yellow filters (λ 465-480). Aqueous humor samples were collected after anesthesia.

3：5 ± 5 kg were used. They were kept in individual cages with free access to food and water, under 12h light/dark cycles or with yellow filters (λ 465-480). Aqueous humor samples were collected after anesthesia. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: TonoVet contact tonometer (Tiolat Oy), HPLC system with Kromaphase C18 column (Scharlau), Waters 1515 Isocratic HPLC pump, 2487 dual absorbance detector, Reodyne injector, Breeze software (Waters), ketamine (Imalgene 1000, Merial), Domtor (DOMTOR?, Orion Pharma), luzindole and 4PPDOT (Tocris), AA92593 (Sigma), yellow filter (λ 465-480).

4：0). Experimental Procedures and Operational Workflow:

4. Experimental Procedures and Operational Workflow: IOP was measured weekly at 10 am using the TonoVet. For pharmacological assays, antagonists were applied topically (20μL), and IOP was measured at intervals. Aqueous humor was extracted with a syringe and 30-gauge needle after anesthesia, stored at -20°C, and processed for HPLC analysis.

5：Data Analysis Methods:

Data were analyzed using ANOVA or student t-test as appropriate, with plots generated using GraphPad Prism software.