研究目的
To assess and compare the accuracy of Kang’s method for ocular magnification correction in circumpapillary retinal nerve fiber layer (cpRNFL) thickness measurement with that of the currently used Littmann’s method.
研究成果
Kang’s method is as accurate as Littmann’s method for global cpRNFL thickness correction in healthy young participants, with no significant difference observed. However, for sectoral corrections, Kang’s method shows high variability and systemic biases, particularly in nasal and inferior sectors, indicating a need for further validation, especially in glaucoma patients.
研究不足
The study included only young healthy participants, limiting generalizability to glaucoma patients. The p value (magnification factor of the imaging system) was assumed based on previous reports and may vary slightly between OCT devices, potentially affecting accuracy. Kang’s method may overestimate cpRNFL thickness in regions with high curvature, such as near the optic disc.
1：Experimental Design and Method Selection:
A prospective study design was used to compare Kang’s and Littmann’s methods for ocular magnification correction in cpRNFL thickness measurement using spectral-domain optical coherence tomography (OCT). Statistical methods included Pearson correlation coefficient and Bland–Altman analysis for accuracy assessment.
2：Sample Selection and Data Sources:
148 eyes of 148 healthy student volunteers from Kitasato University were recruited. Inclusion criteria: corrected visual acuity of 20/20 or better, IOP ≤21 mm Hg, normal optic disc appearance, no fundus disease. Exclusion criteria: image quality ≤70, lack or deviation of B-scan line images.
3：List of Experimental Equipment and Materials:
Spectral-domain OCT (3D OCT-2000, version 8.00; Topcon), noncycloplegic refractometer (KR-8100PA; Topcon), visual acuity chart (Landolt ring), IOP measurement device (NT-530P; NIDEK), axial length measurement device (OA-1000; TOMEY), Microsoft Excel for data analysis.
4：00; Topcon), noncycloplegic refractometer (KR-8100PA; Topcon), visual acuity chart (Landolt ring), IOP measurement device (NT-530P; NIDEK), axial length measurement device (OA-1000; TOMEY), Microsoft Excel for data analysis. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Participants underwent comprehensive ophthalmic examinations. cpRNFL was measured with and without Littmann’s correction using OCT. Kang’s method correction was applied post-measurement using Excel based on axial length and uncorrected cpRNFL thickness. Measurements were performed by two examiners without mydriatic agent. Repeatability was assessed with intra- and interexaminer measurements.
5：Data Analysis Methods:
Data were analyzed using MedCalc software (version 13.2.0.0). Pearson correlation coefficient and Bland–Altman analysis were used to compare methods. Fixed and proportional biases were evaluated, and limits of agreement were calculated.
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