研究目的
To develop a high-sensitivity endoscopic Cerenkov luminescence imaging (ECLI) system for detecting deep tumors and guiding tumor resection in vivo, particularly for hepatocellular carcinoma (HCC).
研究成果
The developed ECLI system with dual-mode cooling achieved high spatial resolution (62.5 μm) and sensitivity (6.29×10-2 kBq/μl 18F-FDG), effectively detecting and guiding resection of deep tumors in mouse models. It shows promise for clinical applications in precise tumor surgery and optical imaging with novel nanoprobes, though further improvements in temporal resolution and biocompatibility are needed.
研究不足
The system may be limited by movement from living organs and tissues, which could affect temporal resolution. The use of 18F-FDG has lower energy compared to other radionuclides, potentially limiting sensitivity. Clinical translation may require further optimization for human use, including biocompatibility of materials and improved device connections.
1：Experimental Design and Method Selection:
The study designed an ECLI system using a clinical laparoscope and EMCCD with a dual-mode cooling approach to enhance sensitivity. Methods included in vitro characterizations (FOV, distortion, resolution, linearity, sensitivity) and in vivo imaging on mouse models.
2：Sample Selection and Data Sources:
Subcutaneous and orthotropic HCC mouse models (n=6 each) were established using HepG2-Red-Fluc cells. Data sources included optical images from the ECLI system and IVIS system for comparison.
3：List of Experimental Equipment and Materials:
EMCCD (DU888+, Andor), laparoscope (WA53000A, Olympus), calibration targets (NT58-403, Edmund Optics), 1951 USAF resolution test board, Eppendorf tubes, 18F-FDG radiopharmaceutical, cell culture materials (DMEM, FBS, penicillin), matrigel, anesthesia (sodium pentobarbital, isoflurane).
4：Experimental Procedures and Operational Workflow:
System setup with cooling to -95°C, parameter setting, animal anesthesia, placement on platform, image acquisition in dark chamber. For in vitro studies, imaging of phantoms and EP tubes with 18F-FDG; for in vivo, injection of 18F-FDG, imaging after 40 minutes, and guided resection.
5：Data Analysis Methods:
Used Prism software for statistical analysis (mean ± SD), ROI analysis for signal intensity and TNR calculations, linear regression for correlations, and median filtering for noise reduction.
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