1：Experimental Design and Method Selection:

The study used reactive magnetron sputtering to deposit nitrogen-doped titanium dioxide thin films on PLLA electrospun microfibers scaffolds. The methodology included optical spectroscopy, SEM, XPS, DFT calculations, and cell culture assays to analyze the coatings and their effects.

2：Sample Selection and Data Sources:

PLLA scaffolds were produced using a 3 wt% solution of PLLA in trichloromethane via electrospinning with specific parameters. Human umbilical vein endothelial cells (HUVEC) were used for cell interaction studies.

3：List of Experimental Equipment and Materials:

Equipment included a balanced magnetron sputtering system, OCEAN OPTICS HR 2000+ spectrometer, Quanta 200 3D SEM, Escalab 250Xi XPS machine, GuavaEasyCyte8 flow cytometer, CO2 incubator, and Axio Observer fluorescence microscope. Materials included PLLA PL-38, trichloromethane, nitrogen gas, titanium target, and various chemicals for cell culture and staining.

4：Experimental Procedures and Operational Workflow:

Scaffolds were prepared, solvent removed under vacuum, and then treated with magnetron sputtering at different times (2, 4, 6, 8 min). Coatings were characterized using optical spectroscopy, SEM, XPS, and DFT. Cell adhesion and proliferation were assessed through fluorescence microscopy after 72 hours of culture.

5：Data Analysis Methods:

Data were analyzed using SpectraSuite, ImageJ, Origin 9.0, Jaguar software for DFT, and Statistica 7.0 for statistical analysis with one-way ANOVA.