研究目的
To investigate the role of reflectance confocal microscopy and optical coherence tomography in diagnosing nodular cystic basal cell carcinoma.
研究成果
Reflectance confocal microscopy and high-definition optical coherence tomography enabled the in vivo diagnosis of nodular cystic basal cell carcinoma, even when dermoscopy was non-specific. OCT was particularly useful for identifying deep cystic components inaccessible to RCM and OCT HD. These non-invasive imaging methods can aid in histological typing and influence therapeutic decisions, such as opting for surgery in this case.
研究不足
The study is limited to a single case report, which may not be generalizable. Technical difficulties were noted with using the Vivascope 1500? camera for mosaics in the eyebrow topography. Depth limitations exist: RCM and OCT HD have reduced effectiveness beyond 200 μm and 300 μm in depth, respectively, while OCT can explore up to 2 mm but has lower cellular resolution (15 μm).
1：Experimental Design and Method Selection:
The study used a case report design involving a 54-year-old male patient with a nodular tumor on the right eyebrow. Non-invasive imaging techniques including dermoscopy, optical coherence tomography (OCT), high-definition OCT (HD-OCT), and in vivo reflectance confocal microscopy (RCM) were employed to diagnose the lesion, with histological examination as the gold standard for confirmation.
2：Sample Selection and Data Sources:
A single patient case was selected based on clinical presentation of a nodular, firm, pinkish tumor measuring 8x8 mm, acquired over approximately 3 years. Data were obtained from clinical examination and imaging.
3：List of Experimental Equipment and Materials:
Equipment included OCT (Vivosight?; Michelson, Maidstone, UK), OCT HD (Skintell?; Agfa, Antwerp, Belgium), and in vivo confocal microscopy (Vivascope 3000?; Caliber Inc, Rochester, NY, USA, distributed by Mavig, Munich). Materials involved standard dermatological and ophthalmological tools for examination.
4：Experimental Procedures and Operational Workflow:
Clinical and dermoscopic examinations were performed first. Then, OCT and OCT HD scans were conducted to assess deeper structures, followed by RCM for cellular-level imaging. Histological examination of the excised lesion was done post-surgery to confirm the diagnosis.
5：Data Analysis Methods:
Imaging data were analyzed visually for characteristic features such as reflective lobules, non-reflective arcs, and cystic structures. Histological analysis used Hematoxylin-Eosin-Safran staining to identify cellular patterns and confirm the diagnosis.
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