1：Experimental Design and Method Selection:

The study involved synthesizing ZnO nanoparticles via reflux and attaching them to COOH-MWNTs using sonication at varying times (60-240 min) to optimize composite disentanglement. Raman spectroscopy was used to monitor the G band intensity as an indicator of disentanglement, and electrochemical methods (cyclic voltammetry and chronoamperometry) were employed to assess UA detection performance.

2：Sample Selection and Data Sources:

Samples included ZnO/COOH-MWNT composites prepared with different sonication times. Uric acid and other analytes (dopamine, acetaminophen, etc.) were used for selectivity tests, sourced from Sigma-Aldrich.

3：List of Experimental Equipment and Materials:

Equipment included a potentiostat (WaveNano?, Pine Instruments), Raman spectrometer (Enwave Optronics ProRaman-L), XPS spectrometer (Perkin-Elmer PHI 560), TEM microscope (Hitachi H-7650), and various chemicals (e.g., COOH-MWNTs from Nanolab, Inc., UA from Sigma-Aldrich).

4：Experimental Procedures and Operational Workflow:

ZnO NPs were synthesized by refluxing Zn(NO3)2·6H2O with NaOH, then mixed with COOH-MWNTs in ethanol and sonicated for specified times. Composites were drop-casted onto glassy carbon electrodes, coated with Nafion, and dried. Electrochemical measurements were performed in a three-electrode cell with PBS buffer, deoxygenated with N2 gas. Raman and XPS analyses were conducted on composite samples.

5：Data Analysis Methods:

Data were analyzed using Randles-Sevcik equation for cyclic voltammetry to determine electroactive surface areas, and Raman G band integrated areas were quantified to correlate with disentanglement. Statistical analysis included linear regression for calibration curves.