1：Experimental Design and Method Selection:

The study involved synthesizing MnO2-mSiO2 nanohybrids via a one-pot method, growing gold nanoshells via seed-mediated growth, and modifying with hyaluronic acid-dopamine complex. Methods included characterization using TEM, SEM, DLS, UV-vis, FT-IR, NMR, ICP-AES, and various imaging and therapeutic assessments.

2：Sample Selection and Data Sources:

Murine breast cancer 4T1 cells were used for in vitro studies, and female nude and Kunming mice with 4T1 xenograft tumors for in vivo studies. Data were sourced from experimental measurements and imaging systems.

3：List of Experimental Equipment and Materials:

Equipment included TEM (JEM-100CX), SEM (SU8020), DLS (Zetasizer Nano ZS), UV-vis spectrophotometer (Cary 60), FT-IR spectrophotometer (TESOR-27), NMR spectrometer (AVANCE III TM HD 400 MHz), ICP-AES (7700x), laser (LASERGLOW Technologies), digital thermometer (TASI-8620), infrared camera (TI400), MSOT imaging system, CT scanner (Gamma Medical-Ideas), MR scanner (Magnetom Trio with Tim), and flow cytometer (MerckMillipore). Materials included chemicals like KMnO4, CTAB, TEOS, HAuCl4, APTES, EDC, HA, MTT, and cell culture reagents.

4：Experimental Procedures and Operational Workflow:

Synthesis steps involved preparing MNHs, Au nanoshells, HA-DN complex, and MAHNPs. In vitro tests included cytotoxicity, hemolysis, hypoxia evaluation, and imaging. In vivo tests involved tumor modeling, imaging (MSOT, CT, MR), blood circulation, biodistribution, and therapeutic efficacy assessments with PTT and RT.

5：Data Analysis Methods:

Data were analyzed using statistical methods (ANOVA, t-test), ImageJ for image analysis, and software for imaging systems. Photothermal conversion efficiency was calculated using a specific formula.