研究目的
Investigating the therapeutic effects of a specific herbal medicine on a particular disease.
研究成果
In summary, we developed a PFP/QDs hybrid material and constructed a 'two-in-one' platform for discrimination and disinfection of pathogens based on the FRET process. First, discrimination of pathogens utilized the different affinities between the complex and variable compositions of pathogenic surfaces. Upon binding to the PFP/QDs, pathogens dis-assembled the complex and interrupted the FRET to different extent, therefore allowing detection of the pathogens by their distinct fluorescence responses. Intensity changes measured from separate channels were processed by LDA that provides efficient and accurate classification of pathogens. Second, the disinfection capitalized on the phototoxicity of the PFP/QDs, especially the PFP component, which generates massive ROS and kills the pathogens upon light irradiation. This method includes many desirable advantages for antimicrobial applications, including easy operation, readily available materials, reasonably simple equipment, accurate results, and theranostic capabilities. We hope to further improve the pathogen killing efficiency and expand the platform with advanced technologies to protect the public health.
研究不足
The technical and application constraints of the experiments, as well as potential areas for optimization.
1：Experimental Design and Method Selection:
The study utilized fluorescence resonance energy transfer (FRET) between a conjugated polymer (PFP) and quantum dots (QDs) for pathogen discrimination and killing.
2：Sample Selection and Data Sources:
Gram-negative bacteria Escherichia coli (E. coli), Gram-positive bacteria Staphylococcus aureus (S. aureus), and the fungi Candida albicans (C. albicans) were selected for the study.
3：List of Experimental Equipment and Materials:
UV-vis absorption spectra and fluorescence spectra were taken on JASCO V-550 spectrophotometer and Hitachi F-4500, respectively. The images of the plate counting were measured by Bio-Rad system. The size distribution and zeta potential values were obtained by a Nano ZS90 (Malvern, UK). The morphology change of pathogens incubated with PFP, QDs and PFP/QDs were determined using a JEOL JSM-7401F SEM, and Confocal images were taken by a confocal laser scanning microscopy (FV1200-IX83, Olympus, Japan.
4：Experimental Procedures and Operational Workflow:
PFP/QD hybrids solution and various pathogens solution were prepared with aqueous solution. The fluorescence spectra of PFP/QD solution was then collected. Next, PFP/QD hybrids solution were mixed with 100 μL of various pathogens, respectively. Finally, the change in the fluorescence spectra was collected.
5：Data Analysis Methods:
The changes in fluorescence intensities were calculated by reading the two fluorescence numerical channels. Moreover, we took advantage of the SPSS (Statistic Package for Social Science) software for regular linear discriminant analysis (LDA) to improve the accuracy and reliability of pathogens discrimination.
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