研究目的
To design a fluorescent probe for PepT1 receptor-targeted colorectal cancer (CRC) imaging using CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) conjugated with KPV (QD-PAAO-KPV).
研究成果
The QD-PAAO-KPV probe exhibits desirable targeting ability, stability, and biosafety for detecting PepT1 expression in CRC, showing promising prospects for CRC detection.
研究不足
The study focuses on the detection of PepT1 expression in CRC using a specific fluorescent probe, which may limit its applicability to other types of cancers or biomarkers. The in vivo stability and long-term biosafety of the probe require further investigation.
1：Experimental Design and Method Selection:
The study involved the synthesis of CIS/ZnS QDs, their conjugation with KPV to target PepT1, and the evaluation of the probe's targeting ability, stability, and biosafety in vitro and in vivo.
2：Sample Selection and Data Sources:
Human colon adenocarcinoma (Caco-2) cells and human hepatocellular carcinoma cell line (HepG2) were used. Athymic nude mice were used for in vivo studies.
3：List of Experimental Equipment and Materials:
Included copper acetate, indium acetate, zinc acetate, 1-dodecanethiol, 1-octadecene, oleic acid, oleylamine, and others.
4：Experimental Procedures and Operational Workflow:
Synthesis of CIS/ZnS QDs, their solubilization in water with PAAO micelles, conjugation with KPV, characterization, cytotoxicity and stability assays, in vitro cellular uptake studies, and in vivo fluorescent imaging.
5：Data Analysis Methods:
Flow cytometry, confocal laser scanning microscopy, and statistical analysis using one-way ANOVA and Student’s t-test.
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