研究目的
To develop a biocompatible quantum dot-antibody conjugate for cell imaging, targeting, and fluorometric immunoassay by improving labeling efficiency and reducing toxicity.
研究成果
The developed QD bioconjugate offers a promising platform for biocompatible cell labeling and antibody quantification in immunoassays, with retained fluorescence properties and specific targeting ability.
研究不足
The study focuses on the specific application of QD bioconjugates for cell imaging and immunoassay, and the long-term stability and potential cytotoxicity in vivo were not extensively explored.
1：Experimental Design and Method Selection:
QDs were functionalized with BSA via a chemical linker, and anti-human immunoglobulin antibodies were oxidized by sodium periodate for conjugation with BSA-modified QDs.
2：Sample Selection and Data Sources:
Recombinant Chinese hamster ovary (CHO) cells expressing human IgG were used for cell-labeling efficiency evaluation.
3：List of Experimental Equipment and Materials:
QDs (CdSe/ZnS), BSA, CDI, sodium periodate, and various cell lines were used.
4：Experimental Procedures and Operational Workflow:
The QD bioconjugate was prepared and characterized using TEM, UV-vis spectroscopy, fluorescence spectrometry, FTIR spectroscopy, and zeta potential measurements. Cell-labeling efficiency was evaluated using confocal microscopy.
5：Data Analysis Methods:
Fluorescence intensity and cell labeling efficiency were quantified using image analysis software.
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