研究目的
To develop versatile fluorescent polymeric systems for biological/biomedical applications by exploring alternative strategies for the design and fabrication of easily available fluorescent systems, focusing on the marriage of supramolecular polymers and fluorescent imaging.
研究成果
The study successfully demonstrated the fabrication of fluorescent supramolecular polymersomes based on water-soluble pillararene/paraquat molecular recognition. These polymersomes exhibited pH-responsive behavior and were capable of encapsulating and releasing doxorubicin in a controlled manner. The aggregation-induced emission of tetraphenylethylene groups provided intense fluorescence, making these polymersomes promising for self-imaged drug delivery applications. The research paves a new way to combine polymer science with supramolecular chemistry to construct functional materials.
研究不足
The study focuses on the pH-responsive behavior of the supramolecular polymersomes and their application in drug release. However, the stability of these polymersomes under physiological conditions and their biocompatibility in vivo were not extensively investigated. Additionally, the efficiency of drug encapsulation and release could be further optimized.
1：Experimental Design and Method Selection:
The study utilized water-soluble pillararene/paraquat molecular recognition to prepare amphiphilic polypseudorotaxanes, which self-assemble into supramolecular polymersomes in water. The methodology included the synthesis of polymer PSPT with pendent paraquat units and tetraphenylethylene (TPE) groups, and the preparation of water-soluble pillar[5,6]arenes WP5 and WP
2：Sample Selection and Data Sources:
The samples included polymer PSPT, water-soluble pillar[5,6]arenes WP5 and WP6, and model guest MC. Data were acquired through proton NMR spectra, gel permeation chromatography (GPC), transmission electron microscopy (TEM), dynamic light scattering (DLS), and confocal laser scanning microscopy (CLSM).
3：List of Experimental Equipment and Materials:
The materials included styrene, compound 2, 4-vinylbenzyl chloride, azobisisobutyronitrile (AIBN), and doxorubicin (DOX). Equipment used included NMR spectrometers, GPC, TEM, DLS, and CLSM.
4：Experimental Procedures and Operational Workflow:
The synthesis of polymer PSPT involved free radical polymerization. The formation of polypseudorotaxanes PR5 and PR6 was achieved by mixing PSPT with WP5 or WP6 in water. The self-assembly behavior was studied by TEM, DLS, and CLSM. The pH-responsive behavior was investigated by adjusting the solution pH and observing the changes in vesicle formation.
5：Data Analysis Methods:
The data were analyzed using proton NMR spectra, GPC data, TEM images, DLS results, and fluorescence spectroscopy to determine the critical aggregation concentration (CAC) and drug release efficiency.
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