研究目的
To highlight the applications of in situ CLSM imaging of supramolecular nanofibers and hydrogels, emphasizing its power in evaluating dynamic chemical processes in stimulus-responsive supramolecular hydrogels.
研究成果
CLSM imaging is one of the most powerful methods available for evaluating dynamic chemical processes that take place in stimulus-responsive supramolecular hydrogels. In situ imaging-based materials design is a promising way to rationally design and evaluate multicomponent supramolecular systems. Confocal fluorescent imaging and the related microscopic techniques will provide opportunities to investigate complex life-inspired chemical systems.
研究不足
Despite its usefulness, CLSM imaging generates artifacts that could lead to misinterpretation of experimental results: (1) photobleaching can occur in long-time imaging, (2) local temperature increases under laser irradiation, and (3) fluorescent crosstalk can occur in a multicolor imaging. Appropriate control experiments should be always conducted.
