研究目的
To develop a quantum dots (QDs)-based nanoprobe for multi-enzyme detection and monitoring enzyme activity in situ using capillary electrophoresis (CE) with bent capillaries.
研究成果
The developed QDs-based nanoprobe combined with CE and FRET technology in bent capillaries effectively detected and monitored the activity of two enzymes (PSP and thrombin). The method demonstrated sensitivity to enzyme concentration, capillary bends, injection time, and applied voltage, offering a novel approach for in-situ multi-enzyme detection with potential applications in disease diagnosis and treatment.
研究不足
The study's limitations include the need for optimization of capillary bends, injection times, and applied voltages to enhance enzymatic cleavage efficiency. Additionally, the method's applicability to other enzymes and its scalability for clinical diagnostics require further investigation.
1：Experimental Design and Method Selection:
The study involved the development of a QDs-based nanoprobe for enzyme detection using CE with bent capillaries. The nanoprobe was designed to include QDs, Cy5, His-tag, and two enzyme cleavage sequences.
2：Sample Selection and Data Sources:
The nanoprobe was prepared by mixing QDs and Cy5-LEVLVP peptide, followed by injection into capillaries with different bends. Enzymes (PSP and thrombin) were then introduced to cleave the peptide.
3：List of Experimental Equipment and Materials:
Included a home-built CE system, high voltage supply, fluorescence microscope, fiber optic spectrometer, and various chemicals and peptides.
4：Experimental Procedures and Operational Workflow:
The process involved preparing the nanoprobe, injecting it into bent capillaries, introducing enzymes, and monitoring fluorescence changes due to FRET.
5：Data Analysis Methods:
Fluorescence intensity changes at 605 nm (QDs donor) and 665 nm (Cy5 receptor) channels were recorded and analyzed to assess enzyme activity.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
