研究目的
Investigating the use of aluminum foil as a substrate for metal enhanced fluorescence of bacteria labeled with quantum dots, to achieve very large enhancement and high contrast.
研究成果
Aluminum foil, along with aluminum and gold films, demonstrated very high metal enhanced fluorescence for bacteria labeled with CdSeS/ZnS quantum dots, with enhancement factors up to 500 and high contrast. Aluminum foil is a cost-effective and reproducible substrate for such applications.
研究不足
The study found that CdTe QDs and silver film substrates did not show significant enhancement or contrast, likely due to toxicity and antibacterial activity, respectively.
1：Experimental Design and Method Selection:
The study utilized confocal HORIBA Labram Raman microscope for fluorescence measurements with excitation wavelengths of 532 nm and 633 nm.
2：Sample Selection and Data Sources:
E. coli bacteria were labeled with CdSeS/ZnS and CdTe quantum dots and placed on aluminum foil, aluminum film, gold film, silver film, and glass substrates.
3：List of Experimental Equipment and Materials:
CdSeS/ZnS alloyed QDs, CdTe core-type QDs, gold, silver and aluminum films, aluminum foil, microscope glass slides.
4：Experimental Procedures and Operational Workflow:
Bacteria were grown, labeled with QDs, and fluorescence measurements were performed.
5：Data Analysis Methods:
Enhancement factors and contrast ratios were calculated from fluorescence signals.
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