研究目的
To develop a pH-responsive nano drug delivery system (NDDS) based on ZnO quantum dots (QDs) modified with zwitterionic polymers for controlled release of drugs and synergistic cancer treatment.
研究成果
The ZnO@P(CBMA-co-DMAEMA) NDDS demonstrated excellent fluorescence properties for cell imaging and drug delivery, with a sensitive pH-responsive charge switch that enhances blood circulation time and promotes endocytosis. The system achieved a high drug loading content and synergistic cancer treatment through the combined effects of Zn2+ and DOX. This NDDS represents a promising approach for targeted cancer therapy.
研究不足
The study is limited by the potential cytotoxicity of ZnO QDs and the need for further in vivo testing to validate the efficacy and safety of the NDDS. The pH sensitivity range may also limit the applicability to tumors with specific extracellular pH values.
1：Experimental Design and Method Selection:
The study involved the synthesis of ZnO@P(CBMA-co-DMAEMA) nanoparticles using a sol-gel method, with the aim of creating a pH-responsive NDDS. The methodology included the modification of ZnO QDs with zwitterionic polymers to enhance stability and drug loading capacity.
2：Sample Selection and Data Sources:
ZnO QDs were synthesized and modified with varying ratios of PCBMA and PDMAEMA. The drug loading and release properties were evaluated using Doxorubicin (DOX) as a model drug.
3：List of Experimental Equipment and Materials:
Materials included zinc chloride, triethylene glycol, ethanol, lithium hydroxide, methacrylic acid, 2,2-azobisisobutyronitrile, and Doxorubicin. Equipment used included a Fourier transform infrared spectrophotometer, UV-Vis spectrophotometer, spectrofluorophotometer, particle size analyzer, high-resolution transmission electron microscope, and X-ray diffractometer.
4：Experimental Procedures and Operational Workflow:
The synthesis involved dissolving Zn(MAA)2 in TEG, adding CBMA, DMAEMA, and AIBN, followed by LiOH·H2O and additional AIBN. The solution was dialyzed against deionized water. Drug loading and release experiments were conducted at different pH values.
5：Data Analysis Methods:
The drug loading content was calculated using UV-vis absorbance. The release of DOX was monitored by fluorescence emission spectroscopy. Cytotoxicity was assessed via MTT assay.
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