1：Experimental Design and Method Selection:

The study employs a spray-assisted coil–globule transition method for encapsulating CPB PQDs into PMMA nanospheres. This method involves synthesizing PVP-capped CPB PQDs via the ligand-assisted reprecipitation method in dichloromethane, dissolving PMMA in the precursor solution, and spraying the mixture into petroleum ether under high-pressure N2 to form nanoscale composite spheres.

2：Sample Selection and Data Sources:

CPB PQDs are synthesized and encapsulated into PMMA nanospheres. The study uses HeLa cells for cytotoxicity tests and live cell imaging.

3：List of Experimental Equipment and Materials:

The materials include CsBr, PbBr2, oleic acid (OA), oleyl amine (OAm), polyvinylpyrrolidone (PVP), N,N-dimethylformamide (DMF), dichloromethane (DCM), PMMA, and petroleum ether (PET). Equipment includes a high-pressure spray nebulizer, transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray diffraction (XRD), and laser confocal microscopy.

4：Experimental Procedures and Operational Workflow:

The procedure involves synthesizing CPB PQDs, encapsulating them into PMMA nanospheres via spray-assisted precipitation, and characterizing the resulting nanospheres for optical properties, stability, and biocompatibility.

5：Data Analysis Methods:

The study analyzes the morphology, size distribution, optical properties, and cytotoxicity of the CPB@PMMA nanospheres using TEM, SEM, XRD, and confocal microscopy.