研究目的
To develop a near-infrared (NIR) emission fluorescent small-molecule probe for the detection and imaging of NAD(P)H in living cells and in vivo.
研究成果
The DCI-MQ probe exhibits high selectivity, sensitivity, and low cytotoxicity, making it an effective tool for imaging endogenous NAD(P)H in living cells and in vivo. It provides a significant contribution to the study of NAD(P)H-related physiological and pathological processes.
研究不足
The study focuses on the development and initial application of the DCI-MQ probe, with potential limitations in the depth of in vivo imaging and the specificity under all physiological conditions.
1：Experimental Design and Method Selection:
The probe DCI-MQ was designed using dicyanoisophorone as the NIR chromophore and quinolinium moiety as the NAD(P)H recognition unit. The synthesis involved three steps and was characterized by nuclear magnetic resonance and high-resolution mass spectrometry.
2：Sample Selection and Data Sources:
The optical properties of DCI-MQ were measured in PBS buffer with and without NADH.
3：List of Experimental Equipment and Materials:
UV-Vis absorption and fluorescence emission spectra were collected using standard laboratory equipment.
4：Experimental Procedures and Operational Workflow:
The probe's response to NADH was tested in vitro, and its application in living cells and tumor-bearing mice was evaluated.
5：Data Analysis Methods:
The fluorescence intensity changes were analyzed to determine the probe's sensitivity and selectivity towards NAD(P)H.
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