研究目的
Developing a novel fluorescent turn-on probe for monitoring exogenous and endogenous H2O2 levels in biological samples and hypoxic cancer diagnosis.
研究成果
The novel fluorescent probe HCyHP demonstrates high selectivity, sensitivity, and biocompatibility for detecting H2O2 in biological samples and hypoxic cancer cells. Its application in imaging H2O2 levels in live cells and tumor-bearing mice highlights its potential for disease diagnosis and hypoxic cancer cell detection.
研究不足
The probe's response to H2O2 is less sensitive under acidic conditions, and its application is primarily limited to mildly alkaline conditions. Future work aims to develop ratiometric and near-infrared H2O2 probes for more precise mapping of H2O2 levels in hypoxic tumor cells.
1：Experimental Design and Method Selection:
The study involved the synthesis of a novel fluorescent probe (HCyHP) through a simple two-step process for the detection of H2O2. The probe's response to H2O2 was evaluated through absorption and emission spectra analysis.
2：The probe's response to H2O2 was evaluated through absorption and emission spectra analysis.
Sample Selection and Data Sources:
2. Sample Selection and Data Sources: Biological samples and hypoxic cancer cells were used to test the probe's efficacy in detecting H2O2 levels.
3：List of Experimental Equipment and Materials:
The probe HCyHP was synthesized and characterized using 1H NMR, 13C NMR, and high-resolution mass spectrometry (HRMS).
4：Experimental Procedures and Operational Workflow:
The probe's sensitivity and selectivity towards H2O2 were tested under various conditions, including different pH levels and in the presence of other biologically relevant substances.
5：Data Analysis Methods:
The fluorescence intensity and absorption spectra were analyzed to determine the probe's response to H2O2, with statistical techniques used to calculate detection limits and linear relationships.
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